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Effect of YM155 on Apoptosis and Autophagy of K562 Cells / 中国实验血液学杂志
Article de Zh | WPRIM | ID: wpr-259582
Bibliothèque responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This study was purposed to investigate the effect of YM155, a survivin inhibitor, on the apoptosis and autophagy of K562 cells.</p><p><b>METHODS</b>K562 cells were treated with YM155 at different concentration. Cell survival was analyzed by CCK-8 assay, the cell apoptosis was detected by flow cytometry. Survivin, BCL-2 and beclin1 mRNA expressions were determined by RT-PCR. Survivin, BCL-2, caspase-3, PARP and LC-3 protein expressions were assayed by Western blot.</p><p><b>RESULTS</b>YM155 inhibited the proliferation of K562 cells in a time- and dose-dependent manners. With the increasing of YM155 concentration and prolonging of action time, the expression levels of mRNA and protein of survivin and BCL-2 decreased, while the expression levels of caspase-3, PARP, beclin1 and LC-3 increased. Compared with the YM155 group, the protein levels of LC-3 and caspase-3 were lower in YM155 combined with 3-MA group.</p><p><b>CONCLUSION</b>YM155 can inhibit K562 cell proliferation by inducing apoptosis and autophagy, while autophagy induction effect can enhance its cytotoxic effect.</p>
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Autophagie / Naphtoquinones / Apoptose / Cellules K562 / Prolifération cellulaire / Cytométrie en flux / Imidazoles Limites du sujet: Humans langue: Zh Texte intégral: Journal of Experimental Hematology Année: 2015 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Autophagie / Naphtoquinones / Apoptose / Cellules K562 / Prolifération cellulaire / Cytométrie en flux / Imidazoles Limites du sujet: Humans langue: Zh Texte intégral: Journal of Experimental Hematology Année: 2015 Type: Article