Construction and identification of helper-dependent adenoviral vector encoding hemagglutinin protein of swin-origin influenza virus A H1N1 / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 157-160, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-259677
ABSTRACT
Abstract In order to investigate immune protection against swine-origin influenza virus (S-OIV) A H1N1, the helper-dependent adenovirus vector (HDAd) system was exploited to construct recombinant HDAd encoding hemagglutinin (HA). The HA gene was synthesized and cloned to the HDAd backbone. Then, the HDAd/HA DNA molecules were transfected into 293Cre4 cells with calcium phosphate. The cells were infected by helper virus 16 hours after the transfection. The 293Cre4 cells were coinfected with HDAd/HA and the helper virus for large-scale preparation of HDAd/HA. The HDAd/HA was obtained and purified twice with CsCI density ultracentrifugation and observed morphologically under transmission electron microscope, and the expression of HA protein was analyzed with RTPCR. Recombinant HDAd/HA expressing HA protein was successfully constructed which could pave the way for in vivo investigation on immunogenicity and efficacy against S-OIV A H1N1 infection.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Lignée cellulaire
/
Adenoviridae
/
Clonage moléculaire
/
Glycoprotéine hémagglutinine du virus influenza
/
Sous-type H1N1 du virus de la grippe A
/
Vecteurs génétiques
/
Virus assistants
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Biomedical Engineering
Année:
2014
Type:
Article
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