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Radioiodide treatment mediated by adenovirus transfer of human sodium iodide symporter gene into androgen-independent prostate cancer / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 1080-1084, 2010.
Article Dans Chinois | WPRIM | ID: wpr-260934
ABSTRACT
This study sought to probe the feasibility of instituting a radioiodide treatment for androgen-independent prostate cancer by adenovirus transfer of the hNIS gene. A recombinant adenovirus, Ad-CMV-NIS, that expressed the NIS gene under the control of cytomegalovirus (CMV) promoter was constructed. In vitro, after infection with Ad-CMV-NIS,PC-3 prostate cancer cells exhibited an uptake of perchlorate-sensitive iodide, approximately 120 times higher than that exhibited by negative control Ad-CMV-GFP-infected cells. The half-time of efflux was 26.6 min. Clonogenic assays demonstrated that Ad-CMV-NIS-infected cancer cells were selectively killed by exposure to 131I. In vivo, Ad-CMV-NIS infected tumors showed significant radioiodine accumulation (16.30 +/- 8.72)% ID/g at 2h postinjection) with an effective half-life of 5.4h. The tumor could be clearly visualized by 131I scintigraphy. These data indicate that infection with Ad-CMV-NIS is an efficient way to induce radioiodide uptake in vitro and in vivo, thus suggesting that NIS-based gene therapy has the potential for use in androgen-independent prostate cancer.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Tumeurs de la prostate / Radiothérapie / Transfection / Thérapie génétique / Adenoviridae / Symporteurs / Utilisations thérapeutiques / Génétique / Radio-isotopes de l'iode / Métabolisme Limites du sujet: Humains / Mâle langue: Chinois Texte intégral: Journal of Biomedical Engineering Année: 2010 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Tumeurs de la prostate / Radiothérapie / Transfection / Thérapie génétique / Adenoviridae / Symporteurs / Utilisations thérapeutiques / Génétique / Radio-isotopes de l'iode / Métabolisme Limites du sujet: Humains / Mâle langue: Chinois Texte intégral: Journal of Biomedical Engineering Année: 2010 Type: Article