Effects of growth stages and pH value on the expression of autolytic enzyme atIS gene of Streptococcus gordonii / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 80-83, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-261133
ABSTRACT
<p><b>OBJECTIVE</b>This study aimed to detect the difference in the expression levels of autolysin atIS gene of Streptococcus gordonii (S. gordonii) at different growth stages and pH values, as well as to analyze the factors regulating atlS gene expression in S. gordonii.</p><p><b>METHODS</b>S. gordonii wild strains (ATCC 35105) were collected at different growth stages (early exponential phase, mid-exponential phase, late exponential stage, and platform stage) and pH values (pH 7 and pH 5.5), and total RNA was extracted by using a conventional method. Fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to measure the relative mRNA expression of atlS gene, with bacterial 16S rRNA as internal reference, for a comparison of the mRNA levels of atlS gene expression in S.gordonii at different growth stages and pH values.</p><p><b>RESULTS</b>FQ-PCR results showed that atlS gene expression increased with gradually increasing growth stage under neutral conditions and was higher than that under acidic conditions (P < 0.05).</p><p><b>CONCLUSION</b>The atlS gene expression in S. gordonii is influenced by growth stage and pH value factors.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines bactériennes
/
ARN ribosomique 16S
/
Expression des gènes
/
Streptococcus gordonii
/
Concentration en ions d'hydrogène
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2015
Type:
Article
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