Effects of bcl-2 antisense oligodeoxynucleotide on proliferation and apoptosis of Raji cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 71-73, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-261358
ABSTRACT
<p><b>OBJECTIVE</b>To study the in vitro antitumor activity of bcl-2 fully phosporothioated antisense oligodeoxynucleotide (bcl-2 ASODN) to malignant lymphoblastic cells.</p><p><b>METHODS</b>Proliferation and apoptosis of Raji cells incubated with bcl-2 ASODN were evaluated by MTT assay, flow cytometry (FCM) and electron microscopy, and the level of bcl-2 protein and mRNA expression were assessed by FCM and RT-PCR, respectively.</p><p><b>RESULTS</b>MTT assay demonstrated that bcl-2 ASODN could partially inhibit the growth of Raji cells. After incubated with ASODN for 48 hours, Raji cells exhibited characteristic morphologic changes of apoptosis, including cytoplasm membrane blebbing, chromatin condensation crescents formation and nuclear fragmentation. The apoptosis rate of Raji cells treated with 20 micromol/L bcl-2 ASON for 72 hrs was 43.86% which is significantly higher than that of control (10.05%). The bcl-2 ASODN induced apoptosis of Raji cells was accompanied by declined expression of bcl-2 mRNA, which decreased to 0.88% at 72 hrs and was significantly lower than that of control (79.54%).</p><p><b>CONCLUSION</b>bcl-2 ASODN induced Raji cells apoptosis by downregulating bcl-2 protein.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
ARN messager
/
Cellules cancéreuses en culture
/
Régulation de l'expression des gènes tumoraux
/
Division cellulaire
/
Oligonucléotides antisens
/
Apoptose
/
Protéines proto-oncogènes c-bcl-2
/
RT-PCR
/
Relation dose-effet des médicaments
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2003
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS