Effects of HLA disparity of two umbilical cord blood units on human engraftment in SCID mice / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 624-627, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-261376
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the feasibility and characteristics of human engraftment in HLA disparate cord blood transplantation.</p><p><b>METHODS</b>Two human HLA-haploidentical or HLA-mismatched cord blood units were transplanted into sublethally irradiated severe combined immunodeficiency (SCID) mice. The characteristics of engraftment, hematopoietic and immunological reconstitution between the two groups were compared.</p><p><b>RESULTS</b>Two mixed cord blood units can engraft in SCID mice with donor-recipient chimerism and reconstitute hematopoiesis and immunological functions. No unfavorable factors had been observed. Only one of the two cord blood units which had higher colony forming ability in vitro could engraft in most SCID mice as shown by HLA-DQB(1) gene detection. Two HLA-haploidentical cord blood units were simultaneously engrafted in 3 SCID mice.</p><p><b>CONCLUSION</b>Double HLA-haploidentical or HLA-mismatched cord blood can engraft in SCID mice and reconstitute hematopoietic and immunological functions. HLA disparity has no significant effect on survival and engrafting rate. However, in less HLA disparity group, two cord blood units were prone to engraft simultaneously.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Chirurgie générale
/
Transplantation hétérologue
/
Répartition aléatoire
/
Antigènes CD
/
Analyse de survie
/
Immunodéficience combinée grave
/
Souris SCID
/
Transplantation de cellules souches de sang du cordon
/
Modèles animaux de maladie humaine
/
Allergie et immunologie
Type d'étude:
Essai clinique contrôlé
/
Étude pronostique
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2002
Type:
Article
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