Preparation and characterization of a single chain antibody fragment of mAb SZ-21 against platelets GPIIIa / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 480-482, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-261378
ABSTRACT
<p><b>OBJECTIVE</b>To prepare a single chain antibody (ScFv) of mAb SZ-21 against platelet GPIIIa for its future clinical application.</p><p><b>METHODS</b>The expression vector pET20b-SZ-21ScFv was constructed and the fusion protein was expressed in E. coli BL21 (DE3) PlysS. The activated fusion protein was obtained after a series of purification steps, including cell breakage, inclusion body solubilization, His-bind resin affinity chromatography and protein refolding.</p><p><b>RESULTS</b>The fusion protein yields were up to 21% of the total amount of bacteria protein. The ScFv fragment could inhibit ADP-induced platelets aggregation in a dose-dependent manner in vitro and the maximal inhibition rate was obtained at a concentration of 20 micro g/ml. It also reacted with endothelial cells as detected by flow cytometry. Moreover, the ScFv fragment was able to inhibit the binding of fibrinogen to platelet.</p><p><b>CONCLUSION</b>The SZ-21ScFv fragment had the activity to inhibit platelets aggregation and the binding of fibrinogen to platelet, being potentially useful for the treatment of thrombotic diseases.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Plaquettes
/
Protéines de fusion recombinantes
/
Fibrinogène
/
Fragments d'immunoglobuline
/
Endothélium vasculaire
/
Agrégation plaquettaire
/
Complexe glycoprotéique IIb-IIIa de la membrane plaquettaire
/
Biologie cellulaire
/
Allergie et immunologie
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2002
Type:
Article
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