Changes of protein tyrosine phosphorylation in erythrocyte band 3 glucose-6-phosphate dehydrogenase deficiency / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 565-567, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-261401
ABSTRACT
<p><b>OBJECTIVE</b>To explore the hemolytic mechanism of glucose-6-phosphate dehydrogenase (G6PD) deficient erythrocytes in the view of phosphorylation of membrane protein.</p><p><b>METHODS</b>The alternation of membrane protein phosphorylation and the effect of dithiothreitol (DTT) on protein phosphorylation were analysed by Western blot technique. The activity of phosphotyrosine phosphatase (PTPs) was determined by using p-nitrophenyl phosphate as substrate.</p><p><b>RESULTS</b>Tyrosine phosphorylation of band 3 protein was obviously enhanced in G6PD-deficient erythrocytes. The activity of PTPs was low compared to the normal erythrocytes. The level of phosphotyrosine in G6PD-deficient erythrocytes incubated with DTT was almost the same as in those without DTT. The results were consistent with the activity of PTPs.</p><p><b>CONCLUSIONS</b>PTPs activity reduction and tyrosine phosphorylation enhancement induced by oxidation in G6PD deficiency play an important role in erythrocytes hemolysis. However, the alternation of thiol group is not the only factor affecting the activity of PTPs in G6PD-deficient erythrocytes.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphorylation
/
Tyrosine
/
Protéine érythrocytaire-1 échangeuse d'anions
/
Technique de Western
/
Protein Tyrosine Phosphatases
/
Membrane érythrocytaire
/
Déficit en glucose-6-phosphate-déshydrogénase
/
Métabolisme
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2002
Type:
Article
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