A preliminary study on the early diagnosis of myelodysplastic syndromes / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 307-310, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-261404
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the four techniques for clonal analysis in the early diagnosis of myelodysplastic syndromes (MDS).</p><p><b>METHODS</b>Four techniques for clonal analysis were performed in bone marrow samples from fifty patients with suspected MDS (1) Conventional cytogenetics (CC) for clonal chromosomal abnormalities; (2) BrdU-sister chromatid differentiation (BrdU-SCD) for cell cycle analysis; (3) Fluorescence in situ hybridization (FISH) for trisomy 8; (4) PCR-SSCP for N-ras mutation.</p><p><b>RESULTS</b>The diagnosis of forty-five patients was compatible with FAB criteria of MDS, the other five patients didn't fully meet the FAB criteria. They had either only one lineage dyspoiesis or no any obvious dysplastic features and two of them were diagnosed as suspicious refractory anemia (RA), one as anemia with hypercellular bone marrow and two as chronic aplastic anemia. The results of the four techniques performed in them showed that four patients had clonal karyotype abnormalities, two had prolonged cell cycle, three had trisomy 8 of different proportions, and one had N-ras mutation. Thus, they were all diagnosed as RA.</p><p><b>CONCLUSION</b>The untypical MDS patients can be diagnosed early by examination with combining several clonal analysis techniques.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Syndromes myélodysplasiques
/
Broxuridine
/
Chromatides
/
Aberrations des chromosomes
/
Gènes ras
/
Hybridation fluorescente in situ
/
Analyse cytogénétique
/
Diagnostic
/
Génétique
/
Méthodes
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Adolescent
/
Adulte
/
Adulte très âgé
/
Enfant
/
Enfant d'âge préscolaire
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Chinese Journal of Hematology
Année:
2002
Type:
Article
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