Effects of As(2)O(3) on BCR-ABL protein level and signal transduction in CML cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 360-362, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-261413
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of As(2)O(3) on BCR-ABL protein level and signal transduction in chronic myeloid leukemia (CML) cells.</p><p><b>METHODS</b>Immunoprecipitation, protein tyrosine kinase (PTK) activity assay, real-time Taqman quantitative PCR and Western blot were used.</p><p><b>RESULTS</b>As(2)O(3) downregulated BCR-ABL protein and STAT1 protein of CML mononuclear cells in the concentrations of 1.0 approximately 2.0 micro mol/L and 0.5 approximately 2.0 micro mol/L after 48 h exposure, respectively. However, p27 protein level was not affected. The PTK activity of BCR-ABL protein was also mildly decreased in CML monouclear cells at 60 h. The bcr-abl mRNA level remained unchanged.</p><p><b>CONCLUSION</b>As(2)O(3) downregulats BCR-ABL protein, STAT1 protein, BCR-ABL signal transduction and PTK activity in CML cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphorylation
/
Protein-tyrosine kinases
/
Leucémie myéloïde chronique BCR-ABL positive
/
Transduction du signal
/
Protéines de fusion bcr-abl
/
Cellules K562
/
Génétique
/
Métabolisme
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Chinese Journal of Hematology
Année:
2002
Type:
Article
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