Study on genetic approach in the detection of Cryptosporidium parvum and Giardia lamblia in acquired immunodeficiency syndrome patients / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 884-888, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-261717
ABSTRACT
<p><b>OBJECTIVE</b>To establish genetic method in detecting Cryptosporidium parvum and Giardia lamblia which often coinfected with AIDS patients.</p><p><b>METHODS</b>Cryptosporidium oocysts and Giardia cysts were isolated and purified from fecal samples of the individuals infected with C. parvum and G. lamblia, respectively. Genomic DNAs were extracted. Two pairs of specific primers were designed or synthesized according to the 18S rRNA gene from C. parvum or the triose phosphate isomerase (tim ) gene from G. lamblia. Polymerase chain reaction(PCR) technique was used to amplify the DNA samples from the oocysts and the cysts, and those from the 6 control samples, including Schitosoma japonicum, Toxoplasma gondii , Entamoeba histolytica, Trichinella spiralis, Trichomonas vaginalis and human blood cells. DNA samples from 30 fecal samples of AIDS patients were detected with the same method.</p><p><b>RESULTS</b>One fragment of 500 bp was amplified with the primer of C. parvum, and the other one of 683 bp was amplified with the primer of G. lamblia. Twenty pg and 0.4 pg DNA of C. parvum and G. lamblia could be detected separately. The specificity of these two pairs of PCR primers was confirmed by the failure in the amplification of the control DNA samples. Out of 30 cases of AIDS patients, 7 showed C. parvum positive, while non Giardia was detected.</p><p><b>CONCLUSION</b>Genetic detection method for C. parvum and G. lamblia detection was established which was more sensitive and specific.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ADN bactérien
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
Syndrome d'immunodéficience acquise
/
Giardiase
/
Giardia lamblia
/
Cryptosporidium parvum
/
Cryptosporidiose
/
Diagnostic
/
Génétique
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Epidemiology
Année:
2006
Type:
Article
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