Effects of mesenchymal stem cells on lymphocyte proliferation of sensitized mice in vitro and their action manner / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1209-1213, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-261899
ABSTRACT
This study was purposed to investigate the effects of mesenchymal stem cells (MSC) on lymphocyte proliferation of sensitized mice in vitro and their action manner so as to more understand the possible mechanisms of bone marrow-derived MSC action on the engraftment of hematopoietic stem/progenitor cells in sensitized mice. Bone marrow-derived MSC were cultured by adherent culture method, the MSC or culture supernatant were used as immune cells or immunologic factor, the spleen lymphocytes of sensitized mice were used as effector cells. The phytohemagglutinin (PHA) was used to stimulate the lymphocyte proliferation, the MTT method was used to detect the mixed lymphocyte reaction. The results showed that bone marrow-derived MSC could inhibit the lymphocyte proliferation of sensitized mice, the MSC cultured supernatant also exhibit this effect. Moreover, the inhibitory effect of MSC supernatent increased along with the increase of cell ratio and concentration, while ratio of these two kind of cells was 11, the inhibitory effect was the highest (p < 0.05). It is concluded that the MSC can suppress lymphocyte proliferation of sensitized mice in vitro through cell-cell direct contact or cell-cell indirect interaction.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Rate
/
Cellules de la moelle osseuse
/
Activation des lymphocytes
/
Cellules cultivées
/
Biologie cellulaire
/
Prolifération cellulaire
/
Allergie et immunologie
/
Cellules souches mésenchymateuses
/
Souris de lignée BALB C
/
Souris de lignée C57BL
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2011
Type:
Article
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