Differentiation of human umblical cord mesenchymal stem cells into Leydig cells in the rat testis interstitium: An experimental study / 中华男科学杂志
National Journal of Andrology
;
(12): 680-685, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-262325
ABSTRACT
<p><b>Objective</b>To explore the feasibility of inducing human umbilical cord mesenchymal stem cells (HUMSCs) to differentiate into Leydig cells in the interstitial tissue of the rat testis.</p><p><b>METHODS</b>HUMSCs were obtained by tissue blocks culture attachment and their purity and multi-lineage differentiation ability were verified by flow cytometry and chondrogenic/adipogenic/osteogenic differentiation. Then the HUMSCs were marked by CM-Dil and transplanted into the interstitial tissue of the rat testis. At 4 and 8 weeks after transplantation, the survival and differentiation status of the HUMSCs were observed by immunofluorescence staining and flow cytometry. The suspension of the rat Leydig cells was obtained at 8 weeks for determining the expression of the Leydig cell marker 3β-HSD in the HUMSCs, the cells labeled with CM-Dil were sorted and cultured, and the medium collected after 3 days of culture for measurement of the testosterone level.</p><p><b>RESULTS</b>The expression of the Leydig cell marker CYPllal was not observed in the HUMSCs at 4 weeks but found at 8 weeks after transplantation and the differentiation rate of 3β-HSD was about 14.5% at 8 weeks. CM-Dil labeled cells survived after sorting and testosterone was detected in the medium.</p><p><b>CONCLUSIONS</b>HUMSCs are likely to differentiate into Leydig cells in the interstitium of the rat testis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Testicule
/
Facteurs temps
/
Cordon ombilical
/
Carbocyanines
/
Cholesterol side-chain cleavage enzyme
/
Marqueurs biologiques
/
Différenciation cellulaire
/
Études de faisabilité
/
Biologie cellulaire
/
Cellules souches mésenchymateuses
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2016
Type:
Article
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