The correlation study of PTEN gene expression and Akt phosphorylation in myelodysplastic syndrome / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 470-473, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-262998
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between PTEN gene expression and Akt phosphorylation (p-Akt) in myelodysplastic syndrome (MDS) and to explore the progression of MDS and the mechanism of high risk transformation to acute myeloid leukemia.</p><p><b>METHODS</b>RT-PCR was used to detect the PTEN mRNA expression in leukemia cell lines K562 (as negative control) and Jurkat (as positive control) and 65 MDS and MDS/AML patients. Flow cytometry was used to detect p-Akt in HL-60 and Jurkat cells and 30 MDS patients.</p><p><b>RESULTS</b>(1) K562 cells present PTEN gene expression while Jurkat cells did not. Of 65 MDS and MDS/AML patients, 27 (41.5%) expressed PTEN mRNA, being significantly lower than that in normal group (85.7%) (P < 0.01). (2) Jurkat cell showed high expression (86.9%) of p-Akt, while HL-60 cell as negative control did not express. P-Akt levels of 30 MDS patients were increased (1.35% - 58.23%), being much higher as compared with that of the normal contrast group (0.54% - 2.34%) (P < 0.01). Moreover, with the rate of blast cells increasing, the p-Akt level was rising up. There is a positive correlation (r = 0.93, P < 0.01) between the low expression rate of PTEN and the positive rate of p-Akt.</p><p><b>CONCLUSION</b>The loss of PTEN gene expression is one of the important factors of p-Akt high expression in MDS patients, moreover, it may speed up the progress of the MDS or transformation to acute myeloid leukemia.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphorylation
/
Syndromes myélodysplasiques
/
ARN messager
/
Cellules de la moelle osseuse
/
Cellules HL-60
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Cellules Jurkat
/
Cellules K562
/
Phosphohydrolase PTEN
/
Protéines proto-oncogènes c-akt
/
Métabolisme
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2007
Type:
Article
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