The inhibitory effect of human endostatin gene on tumor growth of tongue squamous cell carcinoma / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 273-276, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-263394
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of transfected human endostatin (hES) gene in tongue squamous cell carcinoma (TSCC) and its inhibitory effects on the growth of tumor cells in vivo.</p><p><b>METHODS</b>Lipofectamine-mediated hES gene was transferred into Tca8113 cells, selected with Blasticidin S; The stable transfected cells were inoculated in BALB/c mice, and then the growth of xenografts was observed. The hES and vascular endothelial growth factor (VEGF) protein expression of xenografts was detected by S-P immuno-histochemical assay. We also detected the microvessel density (MVD) of xenografts with Weidern's method and apoptotic index of the tumor cells by flow cytometry (FCM).</p><p><b>RESULTS</b>The hES protein expression of xenografts in experimental group was significantly higher than that in control group (P < 0.01), while the expression of VEGF protein was on the other way round (P < 0.01). MVD counting of xenografts in experimental group was lower than that in control group (P < 0.01). The mean apoptotic level of the tumor cells in control group was also lower than in experimental group (P < 0.01). In addition, the inhibitory rate to growth of xenografts induced by hES transfection was 78.9%.</p><p><b>CONCLUSIONS</b>hES gene can be transferred into TSCC cells and then induce corresponding protein expression efficiently in xenograft model, resulting in significantly inhibitory effects on the xenografts in vivo.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Tumeurs de la langue
/
Cellules cancéreuses en culture
/
Carcinome épidermoïde
/
Transfection
/
Thérapie génétique
/
Endostatines
/
Génétique
/
Souris de lignée BALB C
/
Souris nude
/
Transplantation tumorale
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Stomatology
Année:
2004
Type:
Article
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