Bioassay of the soluble human tumor necrosis factor receptor I recombinant plasmid expression in vitro / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To detect the expression of recombinant plasmid PcDNA3.1-sTNFRI in vitro and evaluate the bioactivity of expressed sTNFRI.</p><p><b>METHODS</b>CHO cells were transfected with recombinant plasmid PcDNA3.1-sTNFRI by liposome. sTNFRI in cell culture supernatant was detected by ELISA and sTNFRI blockage of TNF-alpha cytotoxicity in L929 cells evaluated by MTT assay.</p><p><b>RESULTS</b>The expression of sTNFRI in transfected cell culture supernatant was higher than control groups (P < 0.001). The expressed sTNFRI could significantly neutralize TNF-alpha cytotoxicity in L929 cells.</p><p><b>CONCLUSIONS</b>These results showed that the recombinant plasmid PcDNA3.1-sTNFRI can be expressed in mammalian cells and the recombinant sTNFRI has biological function.</p>