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Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 284-290, 2002.
Article Dans Chinois | WPRIM | ID: wpr-263608
ABSTRACT
In this study, about 350 bp cDNA fragment was amplified by PCR. After being sequenced, the AE1-c-end gene fragment was cloned into EcoR I-Pst I site of pGADT7 to form AD ends in the yeast two-hybrid system. The recombinant plasmid was transformed into yeast AH109, and the expression in the yeast was observed. The results demonstrate that AE1-c-end was obtained. pGADT7-AE1-c-end has no toxic effect on the yeast. It can serve as a target gene of yeast two-hybrid system.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Plasmides / Levures / Réaction de polymérisation en chaîne / Clonage moléculaire / ADN complémentaire / Techniques de double hybride langue: Chinois Texte intégral: Journal of Biomedical Engineering Année: 2002 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Plasmides / Levures / Réaction de polymérisation en chaîne / Clonage moléculaire / ADN complémentaire / Techniques de double hybride langue: Chinois Texte intégral: Journal of Biomedical Engineering Année: 2002 Type: Article