In vivo digestive stability of soybean β-conglycinin β-subunit in WZS minipigs / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 85-88, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-264616
ABSTRACT
By now, the digestive stability experiments provided by most authoritative organizations are in vitro tests. Evaluating the protein digestive stability with in vivo models should be more objective. The present study aimed to verify the in vivo digestibility of soybean β-conglycinin β-subunit in Wuzhishan (WZS) minipigs. Three minipigs were surgically fitted with O-stomach and T-ileum cannulae and fed with soybean meals. According to SDS-PAGE, the 50 kD fraction of soybean β-conglycinin β-subunit persisted in the gastric fluid until 6 h after feeding, which was detected at 3 h and clearly visible at 4-6 h in the intestinal fluid. Western blot with anti-β-conglycinin β-subunit McAb confirmed it.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Suidae
/
Porc miniature
/
Chimie
/
Protéines de soja
/
Sous-unités de protéines
/
Digestion
/
Antigènes végétaux
/
Protéines de stockage des graines
/
Globulines
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2015
Type:
Article
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