Coumarins from branch of Fraxinus sieboldiana and their antioxidative activity / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1708-1710, 2008.
Article
de Zh
| WPRIM
| ID: wpr-264835
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents from the branch of Fraxinus sieboldiana, and evaluate their antioxidative activity.</p><p><b>METHOD</b>The chemical constituents were isolated and purified by chromatographic techniques over silica gel, macroporous adsorbent resin, Sephadex LH-20, and preparative HPLC. Structures of the compounds were identified by spectroscopic methods. The antioxidant activity was evaluated by Fe(+2)-cystine induced rat liver microsomal lipid peroxidation.</p><p><b>RESULT</b>Eight coumarins were obtained and their structures were elucidated as esculin (1) , esculetin (2), fraxin (3), fraxetin (4), 6, 7-di-O-beta-D-glucopyranosylesculetin (5), scopoletin (6), cleomiscosin D (7) and cleomiscosin B (8). At a concentration of 10(-6) mol x L(-1), compound 4 showed antioxidative activity inhibiting Fe(+2)-cystine induced rat liver microsomal lipid peroxidation with inhibitory rate of 60%.</p><p><b>CONCLUSION</b>Compounds 5, 7 and 8 were obtained from the genus Fraxinus for the first time. Compound 4 showed remarkable antioxidative activity, which was higher than that of VE (35%).</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Scopolétine
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Ombelliférones
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Microsomes du foie
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Spectroscopie par résonance magnétique
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Peroxydation lipidique
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Chimie
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Coumarines
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Spectrométrie de masse ESI
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Fraxinus
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2008
Type:
Article