Analysis of matrine and oxymatrine in Kushensu injection by UPLC/Q-TOF-MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1398-1401, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-264871
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for the determination of matrine and oxymatrine in Kushensu injection by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS).</p><p><b>METHOD</b>The analysis was performed on a Acquity UPLC BEH C18 column (2.1 mm x 100 mm, 1.7 microm) with the gradient elution of 10 mmol x L(-1) amine acetate (pH 8, adjusted by aqueous ammonia) and methanol. The flow rate was set as 0.30 mL x min(-1). The column temperature was maintained at 30 degrees C. The peaks were detected at 210 nm and the injection volume was 2 microL.</p><p><b>RESULT</b>The calibration curves showed good linearity within the test ranges of 0.084-3.36 microg for matrine and 0.086-3.44 microg for oxymatrine, respectively. The mean recoveries were 103.2%, 98.7%, and the RSD were 1.5%, 1.2%, respectively.</p><p><b>CONCLUSION</b>The established method is simple, rapid and sensitive, can be used for the quality control of matrine and oxymatrine in Kushensu injection through the manufacturing process and clinical implement.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Quinolizines
/
Facteurs temps
/
Calibrage
/
Médicaments issus de plantes chinoises
/
Modèles linéaires
/
Chimie
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Sensibilité et spécificité
/
Spectrométrie de masse ESI
Type d'étude:
Etude diagnostique
/
Étude pronostique
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2008
Type:
Article
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