Potential biochemical mechanisms of neuromuscular junction transmission dysfunction induced by organophosphorus insecticides / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 191-193, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-265079
ABSTRACT
<p><b>OBJECTIVE</b>To explore the potential biochemical mechanisms of neuromuscular junction transmission (NMJT) dysfunction induced by organophosphorus insecticides.</p><p><b>METHODS</b>Ten rats were dosed with phoxim (1,144 mg/kg) and 5 of them developed myasthenia. The NMJT function was evaluated by the mean consecutive differences (MCD) measured by stimulation single fiber electromyography (SSFEMG) with the frequency of stimuli at 20 Hz. The activity of Ca(2+)-ATPase, Na(+), K(+)-ATPase, cAMP dependent protein kinase (PKA), Ca(2+)/phospholipid dependent protein kinase (PKC), and tyrosine protein kinase (TPK) were determined.</p><p><b>RESULTS</b>In comparison with the control and non-myasthenic rats, the results in myasthenic rats showed that (1) the MCDs increased; (2) the activities of Ca(2+)-ATPase, Na(+), K(+)-ATPase and PKA decreased and were negatively correlated with MCD; (3) the activities of PKC and TPK increased, and were positively correlated with MCD; (4) the phosphorylation of serine residuals in sarcolemma was weaker and the phosphorylation of tyrosine residuals was stronger.</p><p><b>CONCLUSIONS</b>The NMJT dysfunction is likely associated with the decrease in Ca(2+)-ATPase and Na(+), K(+)-ATPase activity. The acceleration of desensitization and prolongation of resensitization of nicotinic acetylcholine receptors occur following the increase in tyrosine phosphorylation and the decrease in serine phosphorylation induced by OPs poisoning.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Composés organothiophosphorés
/
Protéine kinase C
/
Pharmacocinétique
/
Protein-Serine-Threonine Kinases
/
Calcium-Transporting ATPases
/
Cyclic AMP-Dependent Protein Kinases
/
Électromyographie
/
Toxicité
/
Insecticides
/
Métabolisme
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Année:
2003
Type:
Article
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