Aminoglycoside ototoxicity in three murine strains and effects on NKCC1 of stria vascularis / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 980-985, 2006.
Article
Dans Anglais
| WPRIM
| ID: wpr-265267
ABSTRACT
<p><b>BACKGROUND</b>After establishing a murine model of aminoglycoside antibiotic (AmAn) induced ototoxicity, the sensitivity of AmAn induced ototoxicity in three murine strains and the effect of kanamycin on the expression of Na-K-2Cl cotransporter-1 (NKCC1) in stria vascularis were investigated.</p><p><b>METHODS</b>C57BL/6J, CBA/CaJ, NKCC1(+/-) mice (24 of each strain) were randomly divided into four experimental groups A kanamycin alone; B kanamycin plus 2, 3-dihydroxybenzoate; C 2, 3-dihydroxybenzoate alone; and D control group. Mice were injected with kanamycin or/and 2, 3-dihydroxybenzoate twice daily for 14 days. Auditory brainstem response (ABR) was measured and morphology of cochlea delineated with succinate dehydrogenase staining. Expression of NKCC1 in stria vascularis was detected immunohistochemically.</p><p><b>RESULTS</b>All three strains in groups A and B developed significant ABR threshold shifts (P < 0.01), which were accompanied by outer hair cell loss. NKCC1 expression in stria vascularis was the weakest in group A (A cf D, P < 0.01) and the strongest in groups C and D (P < 0.05). CBA/CaJ mice had the highest sensitivity to AmAn.</p><p><b>CONCLUSIONS</b>Administration of kanamycin established AmAn induced ototoxicity. Kanamycin inhibited the expression of NKCC1 in stria vascularis. 2, 3-dihydroxybenzoate attenuated AmAn induced ototoxicity-possibly by enhancing the expression of NKCC1. Age related hearing loss did not show additional sensitivity to AmAn induced ototoxicity in murine model.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Seuil auditif
/
Strie vasculaire
/
Kanamycine
/
Chimie
/
Cellules ciliées vestibulaires
/
Symporteurs des ions sodium-potassium-chlorure
/
Toxicité
/
Membre-2 de la famille-12 des transporteurs de solutés
/
Souris de lignée CBA
/
Souris de lignée C57BL
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Medical Journal
Année:
2006
Type:
Article
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