Study on plant tissue culture of Cornus officinalis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To set up the optimums of tissue culture for Cornus officinalis.</p><p><b>METHOD</b>A section of stems of the fine varieties of Cornus officinalis were strictly sterilized, cultivated and geminated in medium with addition of different kinds of hormone.</p><p><b>RESULT AND CONCLUSION</b>The WPM medium with 6-BA 2.0 mg.L-1 + ZT 0.1 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for the multiplication of adventitious buds. The WPM medium with 6-BA 1.0 mg.L-1 + ZT 0.1 mg.L-1 + GA3 0.5 mg.L-1 + NAA 0.1 mg.L-1 was the optimum medium for cultivation in strength. The plantlet could root well in 1/2 MS medium with IBA 1.0 mg.L-1 + 6-BA 0.1 mg.L-1.</p>