Aberrant methylation of hMLH1 gene promoter in papillary thyroid cancer and its clinical significance / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 833-836, 2013.
Article
Dans Zh
| WPRIM
| ID: wpr-267445
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the aberrant promoter methylation of hMLH1 gene promoter and its clinical significance in papillary thyroid cancer (PTC).</p><p><b>METHODS</b>methylation of hMLH1 gene promoter in the cancer tissue and matched tumor-adjacent normal tissue of 152 PTC patients were detected by real-time methylation specific PCR (qMSP). The relationship between the methylation of hMLH1 gene promoter and clinicopathological features was analyzed.</p><p><b>RESULTS</b>The methylation rate of hMLH1 gene promoter in cancer tissues was 37.5% (57/152), of which 33 cases were totally methylated and 24 cases were partially methylated. The methylation rate of adjacent normal tissues was 5.3% (8/152)(all were partially methylated). The methylation rate of PTC tissues was significantly higher than that in the tumor-adjacent normal tissue (P < 0.01). The promoter methylation of hMLH1 gene in PTC was significantly correlated with age, size and number of the primary lesion, local invasion, T stage and lymph node metastasis (P < 0.05) , but not correlated with gender and clinical stage (P > 0.05).</p><p><b>CONCLUSION</b>Promoter methylation of hMLH1 gene is a common molecular event in PTC tissue, and it is significantly correlated with the progression of PTC.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Tumeurs de la thyroïde
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Protéines nucléaires
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Carcinomes
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Carcinome papillaire
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Régulation de l'expression des gènes tumoraux
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Facteurs âges
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Régions promotrices (génétique)
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Méthylation de l'ADN
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Protéines adaptatrices de la transduction du signal
Limites du sujet:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
langue:
Zh
Texte intégral:
Chinese Journal of Oncology
Année:
2013
Type:
Article