Preparation of rhIL-11 from fusion protein by using enterokinase / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 903-909, 2008.
Article
de Zh
| WPRIM
| ID: wpr-267862
Bibliothèque responsable:
WPRO
ABSTRACT
The study was aimed to investigate the technological parameters of rhIL-11 preparation from fusion protein by using enterokinase. Fusion expression vector pET32a/IL-11 was transferred into E.coli BL21 (DE3) pLysS and its expression was induced by IPTG, the lysis supernatant of the engineering strain was purified by Ni-NTA resin and then the target rhIL-11 was digested by auto-cleavaged DsbA-EKL-(His)(8). The results showed that after affinity chromatography, Trx-IL-11 was obtained with the yield of 11.25mg/g, the purity of 89.2% and the recovery of 91.8%. Finally the target rhIL-11 was digested and purified to over 95%. In conclusion, the preparation method of rhIL-11 from fusion protein by using enterokinase is simple and feasible with good separation, which can meet industrial requirements.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéines de fusion recombinantes
/
Enteropeptidase
/
Ingénierie des protéines
/
Chimie
/
Interleukine-11
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Escherichia coli
/
Vecteurs génétiques
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Génétique
/
Métabolisme
/
Méthodes
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2008
Type:
Article