Cloning of human PRL-3 gene and construction of its prokaryotic expression vector / 南方医科大学学报
Journal of Southern Medical University
;
(12): 641-643, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-268059
ABSTRACT
<p><b>OBJECTIVE</b>To obtain the entire coding sequence of human PRL-3 gene and construct its prokaryotic expression vector.</p><p><b>METHODS</b>With total RNA extracted from the human colorectal carcinoma cell line SW480 as the template, PRL-3 gene was amplified by RT-PCR with primers designed according to the published sequence of GenBank, and the product was inserted into pGEM-T Easy vector. The recombinant plasmid pGEM-T-PRL-3 was identified by restriction endonuclease analysis and DNA sequencing. After digestion with restriction endonuclease, PRL-3 gene was cloned into the multicloning sites of the prokaryotic expression vector pGEX-4T-1.</p><p><b>RESULTS AND CONCLUSION</b>The entire coding region of human PRL-3 gene was cloned, and the recombinant pGEX-4T-1-PRL-3 vector was successfully constructed and expressed, which may provide the basis for further study of the relationship between human colorectal carcinoma and PRL-3 gene.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines de fusion recombinantes
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Chimie
/
Protein Tyrosine Phosphatases
/
Clonage moléculaire
/
Analyse de séquence d'ADN
/
ADN complémentaire
/
Lignée cellulaire tumorale
/
Électrophorèse sur gel de polyacrylamide
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2007
Type:
Article
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