Analysis of aristolochic acid A from the aerial and underground parts of Asarum by UPLC-UV / 药学学报
Acta Pharmaceutica Sinica
; (12): 221-223, 2008.
Article
de Zh
| WPRIM
| ID: wpr-268142
Bibliothèque responsable:
WPRO
ABSTRACT
To develop an UPLC-UV method for the determination of aristolochic acid A in the aerial and underground parts of Asarum, an AcquityUPLC HSST3 column (50 mm x 2.1 mm ID, 1.8 microm) was used with the mobile phase of acetonitrile-0.1% FA and eluted in gradient mode. The flow rate was 0.5 mL min(-1), the detection wavelength was 251 nm, and the column temperature was 40 degrees C. The calibration curve was linear in the range of 18 - 1450 ng of aristolochic acid A with correlation coefficient of 0. 9999 and the minimum detective concentration was 18.25 ng mL (-1). The method developed is accurate and reproducible, and can be applied in the determination of aristolochic acid A in Asarum.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plantes médicinales
/
Spectrophotométrie UV
/
Chimie
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Sensibilité et spécificité
/
Racines de plante
/
Asarum
/
Parties aériennes de plante
/
Acides aristolochiques
Type d'étude:
Diagnostic_studies
langue:
Zh
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2008
Type:
Article