Directional differentiation of human adipose-derived stem cells into endothelial cells / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 58-62, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-268644
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the possibility of directional differentiation of human adipose stem cells (hADSCs) into endothelial cells (EC), so as to provide seed cells for tissue engineered vessels.</p><p><b>METHODS</b>hADSCs were isolated from human adipose tissue by collagenase digestion, cultured and amplified by adherence to flasks. Then hADSCs were directionally induced to differentiate into EC by a combination of fibronectin (FN), endothelial cells support liquid (EGM2-MV) containing various growth factors and high concentration of VEGF165 (50 ng/ml). Then, the cells morphology, phenotype and function were identified.</p><p><b>RESULTS</b>Highly homologous hADSCs were obtained, and then hADSCs were directionally differentiated into EC. CD31 and CD34, the specific markers for EC, and vascular endothelial growth factor receptor (KDR) were positive by immunohistochemical staining and RT-PCR. In addition, unique Weibel-Palade bodies in EC were observed under transmission electron microscope. Functionally, hADSCs could swallow Dil-Ac-LDL and form tube-like structures in matrigel after endothelial differentiation.</p><p><b>CONCLUSIONS</b>hADSCs can be successfully induced to differentiate into endothelial cells in vitro.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cellules souches
/
Différenciation cellulaire
/
Cellules cultivées
/
Adipocytes
/
Biologie cellulaire
/
Ingénierie tissulaire
/
Cellules endothéliales
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Plastic Surgery
Année:
2011
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS