Gas chromatography-mass spectrometric analysis of Jatropha curcas leaf extracts prepared by supercritical fluid CO2 extraction / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the bioactive components in Jatropha curcas leaves using gas chromatography-mass spectrometry (GC-MS).</p><p><b>METHODS</b>The bioactive components were extracted from J. curcas leaves by supercritical fluid CO2 extraction and analyzed by using GC-MS.</p><p><b>RESULTS</b>Seventy peaks were detected by GC-MS, and 43 compounds were identified (61.43%). Among the identified compounds, 16 had a content of more than 1%, and the total contents of these 16 compounds reached 81.36%. The four most abundant components were 22,23-dihydro-stigmasterol (16.14%), alpha-tocopherol (15.18%), beta-amylin (7.73%) and dotriacontanol (7.02%). The content of gamma-tocopherol reached 2.88% and vitamin E reached 18.06% in the extract.</p><p><b>CONCLUSION</b>J. curcas leaves contain multiple compounds with anti-tumor, anti-virus and antimicrobial activities.</p>