Effect of different dynamic mechanical strain on the proliferation and synthetic function of human periodontal ligament fibroblasts in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 252-256, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-269580
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes in the proliferation and synthetic function of human periodontal ligament fibroblasts (HPDLF) in response to dynamic mechanical strains of different modes, magnitudes and durations.</p><p><b>METHODS</b>Using a 4-point bending system, the effect of dynamic mechanical strains of different modes, magnitudes and durations on the proliferation of HPDLF was investigated by analyzing the cell cycle changes with flow cytometry (FCM), and the total protein level and the activity of alkaline phosphatase (ALP) in HPDLF were assayed by quantitative analysis.</p><p><b>RESULTS</b>The percentage of G(0)/G(1) cell decreased, S phase cells increased, and the proliferation index (PI), total protein level and activity of ALP were augmented significantly in response to dynamic mechanical micro-strains. These changes showed close correlations to the magnitude and duration of the strain. The mode of strain caused significant changes in G(0)/G(1), S, and G(2)/M phase cell percentages as well as the PI, total protein level and ALP activity of the cells. In the gradient strain group, the cell proliferation activity, total protein level and ALP activity were obviously higher than those in 1000 and 4000 microstrain groups.</p><p><b>CONCLUSION</b>The changes in the proliferation and synthetic function of HPDLF are closely correlated to the mode, magnitude and duration of the strains.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Desmodonte
/
Contrainte mécanique
/
Protéines
/
Cycle cellulaire
/
Cellules cultivées
/
Biologie cellulaire
/
Prolifération cellulaire
/
Phosphatase alcaline
/
Fibroblastes
/
Cytométrie en flux
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2010
Type:
Article
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