Effect of recombinant human erythropoietin on hippocampal p-Akt and caspase-9 expressions in rats with status epilepticus and the mechanism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 64-69, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-269625
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of recombinant human erythropoietin (rhuEPO) on p-Akt and caspase-9 expressions in the hippocampus of rats with status epilepticus (SE) and explore the neuroprotective mechanism of rhuEPO.</p><p><b>METHODS</b>Adult male SD rats were randomized into control, PTZ, rHuEPO, LY294002 group, and DMSO groups and treated with normal saline (NS), PTZ, PTZ+rHuEPO, PTZ+LY294002+rHuEPO, and PTZ+DMSO+rHuEPO, respectively. The behavioral and electroencephalogram (EEG) changes of the rats were recorded, and the expressions of p-Akt and caspase-9 were detected using immunohistochemistry. The hippocampal expression of caspase-9 mRNA was detected using RT-PCR, and the expressions of Akt and p-Akt proteins were determined with Western blotting.</p><p><b>RESULTS</b>The p-Akt-positive cell and p-Akt protein expression increased significantly while the caspase-9-positive cell and caspase-9 mRNA expression decreased in rHuEPO group as compared with those in PTZ group (P<0.05). LY294002 treatment prior to rHuEPO injection significantly abolished the effects of rHuEPO on caspase-9 and p-Akt immunohistochemical positivity and caspase-9 mRNA and p-Akt protein expressions (P<0.05).</p><p><b>CONCLUSION</b>Administration of rHuEPO activates the PI3K/Akt signaling pathway in SE rats and increases the expression of p-Akt protein to regulate the expression of caspase-9, a regulatory factor of the mitochondrial-dependent apoptotic pathway, and therefore provides anti-apoptotic and neuroprotective effects.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
État de mal épileptique
/
Protéines recombinantes
/
ARN messager
/
Répartition aléatoire
/
Érythropoïétine
/
Rat Sprague-Dawley
/
Neuroprotecteurs
/
Utilisations thérapeutiques
/
Traitement médicamenteux
/
Protéines proto-oncogènes c-akt
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2010
Type:
Article
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