Cloning and sequence analysis of MYB transcriptional regulator SmP gene of Saussurea medusa Maxim / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 368-371, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-270051
ABSTRACT
A full-length cDNA encoding a MYB-related regulatory gene was isolated from a cDNA library prepared from mRNAs of the red line callus of S. medusa by TD-PCR. The cDNA, designated SmP, is 969 nucleotides long and has an open reading frame of 771 bp with a deduced amino acid sequence of 256 residues. The putative protein of SmP has two typical conversed R2R3-Myb DNA-binding domains in N-terminal and displays a rather high degree of similarity to OsMYB from rice and LBMI from tobacco, showing 73% and 70% identity within the DNA-binding domains. However, the C-terminal domain of the SmP protein does not show obvious similarity to any other known protein sequence. It is rich in hydrophilic amino acids, especially in serine residues (18.38%), partly organized in homopolymeric stretches, a feature often found in activation domain of transcription factors.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Protéines végétales
/
Facteurs de transcription
/
Données de séquences moléculaires
/
Chimie
/
Réaction de polymérisation en chaîne
/
Séquence d'acides aminés
/
Classification
/
Clonage moléculaire
/
Similitude de séquences d'acides aminés
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2003
Type:
Article
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