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Effects of lipopolysaccharide on actin reorganization and actin pools in endothelial cells / 中华创伤杂志(英文版)
Chinese Journal of Traumatology ; (6): 195-200, 2004.
Article Dans Anglais | WPRIM | ID: wpr-270276
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the dose and time-dependent effects of lipopolysaccharide (LPS) on cytoskeletal F-acitn and G-actin reorganizations by visualizing their distribution and measuring their contents in human umbilical vein endothelial cell line ECV-304.</p><p><b>METHODS</b>F-actin was labeled with rhodamine-phalloidin and G-actin with deoxyribonuclease I (DNase I)conjugated with fluorescein isothiocyanate (FITC). Contents of cytoskeletal proteins were obtained by flow cytometry.</p><p><b>RESULTS</b>F-actin was mainly distributed peripherally in endothelial cells under normal conditions. LPS stimulation caused the formation of stress fibers and filopodia. G-actin was normally seen in perinuclear and nuclear areas in control ECV-304 cells. Under LPS stimulation, G-actin dots appeared in the cytoplasmic region. The actin disorganization was accompanied by the time- and dose- dependent decrease in F-actin pool and increase in G-actin pool.</p><p><b>CONCLUSIONS</b>LPS can induce characteristic morphological alterations of actin cytoskeleton and formation of intercellular gap in endothelial cells, accompanied by changes in F-actin and G-actin pools.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phalloïdine / Pharmacologie / Rhodamines / Veines ombilicales / Cellules cultivées / Chimie / Lipopolysaccharides / Analyse de variance / Actines / Fluorescéine-5-isothiocyanate Limites du sujet: Humains langue: Anglais Texte intégral: Chinese Journal of Traumatology Année: 2004 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phalloïdine / Pharmacologie / Rhodamines / Veines ombilicales / Cellules cultivées / Chimie / Lipopolysaccharides / Analyse de variance / Actines / Fluorescéine-5-isothiocyanate Limites du sujet: Humains langue: Anglais Texte intégral: Chinese Journal of Traumatology Année: 2004 Type: Article