Effects of basic fibroblast growth factor on biological characteristics of osteoblasts / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 229-233, 2003.
Article
Dans Anglais
| WPRIM
| ID: wpr-270325
ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the effects of exogenous basic fibroblast growth factor (bFGF) on biological characteristics of rat osteoblasts cultured in vitro.</p><p><b>METHODS</b>The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF (5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 (TGF-beta(1)) was detected to observe the effects of bFGF on growth and differentiation of osteoblasts.</p><p><b>RESULTS</b>bFGF (5-50 ng/ml) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-beta(1) mRNA increased significantly, but the intracellular ALP content decreased.</p><p><b>CONCLUSIONS</b>bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-beta(1), but cannot promote the differentiation of osteoblasts.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Pharmacologie
/
ARN messager
/
Cellules cultivées
/
Facteur de croissance fibroblastique de type 2
/
Facteur de croissance transformant bêta
/
Rat Sprague-Dawley
/
Antigène nucléaire de prolifération cellulaire
/
Phosphatase alcaline
/
Facteur de croissance transformant bêta-1
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Journal of Traumatology
Année:
2003
Type:
Article
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