Effect of magnesium-free on glucocorticoid receptor expression in primary cultured cortical neurons of fetal rats in vitro / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 211-214, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-270386
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes of glucocorticoid receptor (GR) expression in embryonic rat cortical neurons exposed to transient Mg(2+)-free treatment.</p><p><b>METHODS</b>Six days after rat cortical neuronal cultures, two groups were created based on the medium to which were transiently exposed. The control group was exposed to a physiological solution (PS), and the Mg(2+)-free group was exposed to the same medium as the control group except for the removal of magnesium. The expression of GR mRNA and protein was determined by real-time PCR and immunocytochemistry staining 1, 7 and 12 days after transient Mg(2+)-free treatment.</p><p><b>RESULTS</b>Compared to the control group, the Mg(2+)-free group displayed the significantly less accumulated optical density (AOD) of GR immunoreactivity 12 days after transient Mg(2+)-free treatment (p<0.05). On the contrary, GR mRNA expression increased significantly 1 and 7 days after transient Mg(2+)-free treatment in the Mg(2+)-free group (p<0.05).</p><p><b>CONCLUSIONS</b>GR expression is modified following Mg-free-induced injury in cultured developing neurons in rats.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Axe hypophyso-surrénalien
/
ARN messager
/
Récepteurs aux glucocorticoïdes
/
Survie cellulaire
/
Cellules cultivées
/
Cortex cérébral
/
Rat Wistar
/
Foetus
/
Génétique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Contemporary Pediatrics
Année:
2010
Type:
Article
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