S phase cell percentage normalized BrdU incorporation rate, a new parameter for determining S arrest / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 215-219, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-270611
ABSTRACT
In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) incorporation rate, was introduced to detect S arrest. The results showed a positive linear correlation between the BrdU incorporation rate and the S fraction in unperturbed 16HBE cells. Theoretical analysis indicated that only S arrest could result in a decrease in the SFN-BrdU incorporation rate. Additionally, the decrease in SFN-BrdU incorporation rate and the activation of DNA damage checkpoints further demonstrated that S arrest was induced by diethyl sulfate treatment of 16HBE cells. In conclusion, SFN-BrdU incorporation rate can be used to detecting S arrest.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Altération de l'ADN
/
Broxuridine
/
Pharmacocinétique
/
Phase S
/
Biologie cellulaire
/
Prolifération cellulaire
/
Cellules épithéliales
/
Points de contrôle de la phase S du cycle cellulaire
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2014
Type:
Article
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