Patterns of hepatic cells death during hepatic ischemia/reperfusion injury in cirrhotic rats / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 455-459, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-270670
ABSTRACT
<p><b>OBJECTIVE</b>To research on the main pattern of hepatic cells death during hepatic ischemia/ reperfusion (I/R) injury in cirrhotic rat.</p><p><b>METHODS</b>Cirrhotic rat model was established by carbon tetrachloride replication. These rats were randomly divided into sham operation group and I/R group. In the I/R group, 70% i/R injury model was established and then the liver samples were taken 0, 1, 6, 24, and 48 hours after reperfusion. Serum alanine aminotransferase (ALT)/aspartate aminotransferase (AST) levels, Na+ - K+ ATPase, and Ca2+ ATPase were compared. the percentage of apoptotic/oncotic hepatic cells was measured with flow cytometry, and the changes in hepatic cellular structures were observed under transmission electron microscope.</p><p><b>RESULTS</b>Compared with the sham operation group, the levels of serum AST and ALT significantly increased in the I/R group (P < 0.05), reaching their peak levels at the 6th hour. The activities of Na+ - K+ ATPase and Ca2+ ATPase dramatically decreased one hour after reperfusion and then gradually recovered (P < 0.05). Hepatic cells mainly suffered oncosis at the early stage after reperfusion (within 6 hours); at the late stage (around 24 hours after reperfusion), apoptosis became the main death pattern.</p><p><b>CONCLUSION</b>Oncosis is the main pattern of hepatic cells death during I/R injury in cirrhotic rat, and the severity of hepatic injury correlates with the oncosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Aspartate aminotransferases
/
Sang
/
Lésion d'ischémie-reperfusion
/
Répartition aléatoire
/
Rat Wistar
/
Rat Sprague-Dawley
/
Apoptose
/
Alanine transaminase
/
Modèles animaux de maladie humaine
/
Cirrhose du foie
Type d'étude:
Essai clinique contrôlé
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2008
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS