Identification and characterization of the BGR-like gene with a potential role in human testicular development/spermatogenesis / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 21-32, 2005.
Article
Dans Anglais
| WPRIM
| ID: wpr-270877
ABSTRACT
<p><b>AIM</b>To investigate the roles of the BGR-like gene in testicular development/spermatogenesis.</p><p><b>METHODS</b>A human testis cDNA microarray was hybridized with probes from human adult testes and embryo testes. The differentially expressed clones were sequenced and analyzed. Expression of the BGR-like gene was analyzed by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>A new gene exhibiting 50-fold difference in expression level between adult and fetal human testes was cloned and named the BGR-like gene. The cDNA consisted of 2500 nucleotides and had an open reading frame of 1437 nucleotides encoding a putative protein of 497 amino acid residues. Homologous comparison showed that the BGR-like gene was a new alternative splicing variant of the BGR gene and had sequence homology with the bubblegum gene of human, mouse, rat and Drosophila. Protein motif analysis of the BGR-like gene revealed that it contained a conserved adenosine monophosphate (AMP)-binding domain and a fatty acyl-CoA synthetase signature motif which existed in all acyl-CoA synthetases. The BGR-like gene transcript was imperceptibly expressed in human fetal testes, highly in human adult testes and moderately in elderly testes and human Leydig cells. RT-PCR-based tissue distribution experiments showed that the BGR-like gene was exclusively expressed in testes and was a testes-specific isoform of the BGR gene. A BGR-like gene transcript was not detected in some azoospermic testes.</p><p><b>CONCLUSION</b>The BGR-like gene may play an important role in spermatogenesis/testicular development and may be correlated with male infertility.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Spermatogenèse
/
Testicule
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Régulation de l'expression des gènes
/
Séquence d'acides aminés
/
Analyse de séquence d'ADN
/
Épissage alternatif
/
Coenzyme A ligases
/
RT-PCR
Limites du sujet:
Adulte
/
Adulte très âgé
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Asian Journal of Andrology
Année:
2005
Type:
Article
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