Analysis of anisodine and its metabolites in rat plasma by liquid chromatography-tandem mass spectrometry / 药学学报
Acta Pharmaceutica Sinica
;
(12): 518-521, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-271415
ABSTRACT
<p><b>AIM</b>To identify anisodine and its metabolites in rat plasma after ingestion of anisodine by combining liquid chromatography and tandem mass spectrometry (LC-MS(n)).</p><p><b>METHODS</b>Plasma samples from rats after a single orally administration of 20 mg anisodine were added with methanol to precipitate protein. Then, it was analyzed by LC-MS(n). Identification and structural elucidation of the metabolites were performed by comparing their changes in molecular masses, retention-times and full scan MS(n) spectra with those of the parent drug and blank plasma.</p><p><b>RESULTS</b>The results revealed that the parent drug and its four metabolites (norscopine, scopine, hydroxyanisodine, N-oxide anisodine) existed in rat plasma.</p><p><b>CONCLUSION</b>This method is sensitive, rapid, simple, and it is suitable for the rapid identification of drug and its metabolits.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plantes médicinales
/
Dérivés de la scopolamine
/
Chimie
/
Administration par voie orale
/
Chromatographie en phase liquide
/
Sensibilité et spécificité
/
Rat Wistar
/
Solanaceae
/
Spectrométrie de masse en tandem
/
Métabolisme
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2006
Type:
Article
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