Expression of cyclin D1 and p27kip1 in renal cell carcinoma and its significance / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 483-487, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-271498
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of cyclin D1 and p27(kip1) in the occurrence and development of conventional renal cell carcinoma(CRCC).</p><p><b>METHODS</b>RT-PCR and Western-blot were used to detect mRNA and protein contents of cyclin D1 and p27(kip1) in 25 CRCCs and 10 normal renal tissue distant to tumor. Immunohistochemistry was used to investigate the expression of cyclin D1 and p27(kip1) in pathological tissue sections of 76 CRCCs. The relationship between those index and clinicopathological parameters was analyzed statistically.</p><p><b>RESULT</b>In CRCC, the expression of cyclin D1 was higher than that of the control group. The higher cyclin D1 content was related to big tumor size (P<0.05); The expression of p27(kip1) was lower than that of the control group, and the lower p27(kip1) was related to higher nuclear grade and TNM stage (P<0.01). The 5-year cumulative survival rate of the p27(kip1) high expression group is longer than that of the low group (P<0.01).</p><p><b>CONCLUSION</b>The excessive expression of cyclin D1 and lower expression of p27(kip1) play an important role in the carcinogenesis of CRCC. The lower expression of p27(kip1) may affect the progression of the tumors. The detection of p27(kip1) may be as a reference marker in the prognosis of CRCC.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
ARN messager
/
Néphrocarcinome
/
Régulation de l'expression des gènes tumoraux
/
Technique de Western
/
Cycline D1
/
RT-PCR
/
Inhibiteur p27 de kinase cycline-dépendante
/
Génétique
/
Tumeurs du rein
Limites du sujet:
Adolescent
/
Adulte
/
Adulte très âgé
/
Aged80
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2007
Type:
Article
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