Determination of nucleosides in siweilingzhi mixture by HPCE / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for determining nucleosides (adenoside and guanoside) in Siweilingzhi Mixture by HPCE.</p><p><b>METHOD</b>Adenoside and guanoside were separated within 25 min using an 20 mmol.L-1 borate buffer with 30 mmol.L-1 SDS and 5% Ethanol (adjusted to pH 10.0 with sodium hydroxide solution), with an operation voltage of 10 kV, temperature of 20 degrees C and a hydrodynamic injection time of 15 s. Seperations were carried out in a fused-silica capillary 75 microns id x 57 cm (effective length 50 cm) with peak detection by direct UV at 254 nm.</p><p><b>RESULT</b>Regression equation of adenoside and that of guanoside were Y = 0.0705 + 0.01707X (r = 0.9995) and Y = 0.0232 + 0.01864X (r = 0.9999) respectively. The average recovery rate was 99.22% (RSD = 3.66%) and 104.3% (RSD = 1.91%) respectively. Nine samples were determined with the method.</p><p><b>CONCLUSION</b>The method is simple, rapid and accurate with good repeatability and it can be used to determine nucleosides.</p>