RAPD analysis on different varieties of Rehmannia glutinosa / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To provide molecular evidences for its breeding by studying the genetic relationship among varieties of Rehmannia glutinosa.</p><p><b>METHOD</b>Nineteen varieties were detected by Randomly Amplified Polymorphic DNA(RAPD) markers.</p><p><b>RESULT</b>The 20 selected primers produced 163 bands, among which 114(69.9%) were polymorphic. A DNA molecular dendrogram was established based on Hierarchical cluster analysis of 163 DNA bands amplified by 20 primers, which divided the 19 varieties into four groups Group Beijing, Group 85-5, Group Guolimao and the other Group.</p><p><b>CONCLUSION</b>8 varieties of Group Beijing have a close genetic relationship, and so have varieties of Group 85-5, which provides information for Rehmannia glutinosa's breeding.</p>