Effect of GSTP1 and MTHFR Gene Polymorphism on Side Effects of HD-MTX in ALL Children / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To Study the effect of C677T and MTHFR gene polymorphism on side effects of HD-MTX in ALL children.</p><p><b>METHODS</b>The gene polymorphism of C677T A303G and MTHFR C677T were detected by PCR in 98 ALL children from January 2014 to January 2016. The side effects during HD-MTX therapy were observed, and the relationship among GSTP1, MTHFR gene polymorphism and incidence of side effect of HD-MTX were analyzed.</p><p><b>RESULTS</b>Among 98 ALL children, the gene variation was observed in 61 ALL children (62.24%). Polymorphism study on C677T A303G showed that the gene frequency of A was 84.69%, while that of G was 15.31%; for polymorphism of MTHFR C677T, gene frequency of C was 66.33%, and that of T was 33.67%. Seven patients(7.14%) experienced with bone marrow supression, 23 patients(23.47%) with liver function damage, 15 patients(15.31%) with renal function damage, 48 patients(48.98%) with gastrointestinal reactions and 46 patients(46.94%) with mucosal lesions. After adjustment of sex, age, risk stratification and dosage of MTX, the gene polymorphism had no significant relationship with bone marrow suppression, gastrointestinal reactions and mucosal lesions(P>0.05). However, the number of the mutant genes had statistically significant relationship with liver and renal function damage(P<0.05).</p><p><b>CONCLUSION</b>The risk of side effects during HD-MTX therapy increases in ALL children with combined mutation of MTHFR and C677T.</p>