Antisense Deoxyoligonucleotides Inhibit Activities of Matrix Metalloproteinase-2 in Human Fibrosarcoma HT1080 Cells / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 444-449, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-27225
ABSTRACT
PURPOSE:
MMP-2, 72 kDa-type IV collagenase, plays a major role in the migration and growth of tumor cells, a process that requires the disintegration of basement membrane. Activation of MMP-2 is correlated with the invasiveness of various tumors. The aim of this study was to determine the sequence-specific phosphorothioated oligodeoxynucleotides (ODNs) inhibiting the translation of MMP-2 mRNA and the subsequent invasiveness of tumor cells. MATERIALS ANDMETHODS:
Eight types of antisense ODNs were designed and each (8micro gram/ml) were transfected into HT1080 cells. The effects of these antisense ODNs on MMP expression were examined by gelatin zymography, Western blot, Northern blot and matrigel assay.RESULTS:
Antisense-5 (+904~923), antisense-6 (+1274~+1293) and antisense-7 (+1646~+1665) reduced the MMP-2 activity of the culture supernatant in HT1080 fibrosarcoma cells. Treatment with antisense-6 showed inhibition of MMP-2 mRNA and protein, and in vitro invasion in a dose-dependent manner.CONCLUSION:
Antisense-6 might be one of the therapeutic candidates for tumor invasion and metastasis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oligodésoxyribonucléotides
/
Membrane basale
/
ARN messager
/
Technique de Western
/
Technique de Northern
/
Collagenases
/
Matrix metalloproteinase 2
/
Fibrosarcome
/
Gélatine
/
Métastase tumorale
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Cancer Research and Treatment
Année:
2002
Type:
Article
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