Effect of Human Umbilical Cord Mesenchymal Stem Cells on Etoposide-induced Nalm-6 Cell Apoptosis / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 178-183, 2016.
Article
de Zh
| WPRIM
| ID: wpr-272482
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSC) on VP16-induced apoptosis of Nalm-6 cells.</p><p><b>METHODS</b>hUC-MSC were isolated and identified using morphological observation and flow cytometry, then Nalm-6 cells were treated with hUC-MSC with or without VP16. Apoptosis and cell cycle were assayed by FACS. The mRNA levels of apoptosis-related genes BCL-2, BAX and caspase-3 were detected by quantitative RT-PCR, and the protein levels of BCL-2, BAX and caspase-3 were examined by Western blot.</p><p><b>RESULTS</b>FACS showed that hUC-MSC inhibited the proliferation and decreased apoptosis of Nalm-6 cells resulted from VP16. The quantitative RT-PCR showed that hUC-MSC increased the mRNA expression level of BCL-2 and decreased the expression level of BAX and caspase-3 (P < 0.05). Western blot showed that the protein expression level of BCL-2 increased, and expression level of BAX and caspase-3 decreased in Nalm-6 cells after co-culture with hUC-MSC (P < 0.05).</p><p><b>CONCLUSION</b>hUC-MSC may protect Nalm-6 cells from apoptosis induced by VP16 through regulation of BCL-2, BAX and caspase-3.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Cordon ombilical
/
Cycle cellulaire
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Cellules cultivées
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Apoptose
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Techniques de coculture
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Protéines proto-oncogènes c-bcl-2
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Biologie cellulaire
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Lignée cellulaire tumorale
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Protéine Bax
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Étoposide
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2016
Type:
Article