Methylation of miR-378 in Chronic Myeloid Leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 61-65, 2016.
Article
Dans Zh
| WPRIM
| ID: wpr-272504
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the methylation status of miR-378 promoter in chronic myeloid leukemia (CML) and to analyze its clinical significance.</p><p><b>METHODS</b>The unmethylation level of miR-378 gene promoter in bone marrow mononuclear cells of 25 healthy donors and 53 patients with CML was detected by using real-time quantitative methylation-specific PCR (RQ-MSP).</p><p><b>RESULTS</b>The hypomethylation of miR-378 gene promoter was found in 17/53 (32.1%) patients, but only in 1/25 (4.0%) of controls. The difference between the two groups was very statistically significant (P < 0.01). The frequency of miR-378 unmethylation in CML patients at chronic phase (CP), accelerated phase (AP) and blastic phase (BP) was 35.0% (14/40), 40.0% (2/5), and 12.5% (1/8), respectively. However, there were no significant differences in the unmethylation level of miR-378 among CML patients at different sexes, stages and karyotypes. No significant differences could be observed in age, white blood cell counts, platelet count, hemoglobin level and BCR/ABL1 transcript level (P > 0.05). CONCLUDSION: The miR-378 hypomethylation is a common molecular event in CML, especially at chronic or accelerated phases.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Cellules de la moelle osseuse
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Leucémie myéloïde chronique BCR-ABL positive
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Études cas-témoins
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Régions promotrices (génétique)
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Méthylation de l'ADN
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MicroARN
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Génétique
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Métabolisme
Type d'étude:
Observational_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2016
Type:
Article