Identification of Bx02 Alleles and Genetic Analysis of the Involved Ancestry / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1749-1752, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-272528
ABSTRACT
<p><b>OBJECTIVE</b>To identify the genotypes of the blood sample whose blood grouping showed discrepancies and study the ABO alleles' molecular characteristics of the involved ancestry.</p><p><b>METHODS</b>Blood samples were preliminary genotyped by PCR-SSP. Complete exon 6 and 7 in the ABO genes were amplified by PCR and the PCR products were directly sequenced and cloning sequenced to identify its genotype.</p><p><b>RESULTS</b>Sequence analysis indicated that 3 samples of the family had an nt905A>G mutation in the B gene compared with ABO*B101. Combined with the serological results, the propositus could be typed as Bx02/O102.</p><p><b>CONCLUSION</b>DNA sequencing analysis is able to identify the serological phenotype samples that forward and reverse group methods were incongruous.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phénotype
/
Système ABO de groupes sanguins
/
Groupage sanguin et épreuve de compatibilité croisée
/
Séquence nucléotidique
/
Dépistage génétique
/
Réaction de polymérisation en chaîne
/
Exons
/
Allèles
/
Génotype
/
Mutation
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2015
Type:
Article
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