In vitro study on cranial neural crest differentiating into ectomesenchymal cell of the first branchial arch by FGF-8 / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 319-322, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-273227
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8.</p><p><b>METHODS</b>Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.</p><p><b>RESULTS</b>Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.</p><p><b>CONCLUSIONS</b>On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Région branchiale
/
Différenciation cellulaire
/
Cellules cultivées
/
Nerfs crâniens
/
Biologie cellulaire
/
Facteur de croissance fibroblastique de type 8
/
Mésoderme
/
Lignées consanguines de souris
/
Crête neurale
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Stomatology
Année:
2005
Type:
Article
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