Altered nuclear factor-kappaB inducing kinase expression in insulin-resistant mice / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 3646-3651, 2011.
Article
Dans Anglais
| WPRIM
| ID: wpr-273998
ABSTRACT
<p><b>BACKGROUND</b>Insulin resistance is an underlying feature of both type 2 diabetes and metabolic syndrome. Currently, it is unclear whether nuclear factor (NF)-κB inducing kinase (NIK) plays a role in the development of insulin resistance. The present in vivo study investigated the roles of NIK and IκB kinase α (IKKα) in obesity-induced insulin resistance using animal models.</p><p><b>METHODS</b>NIK expression was evaluated by Western blotting in male Lep(ob) mice and C57BL/6J mice fed a high-fat diet (HFD) (45% fat). After metformin and sulfasalazine treatment, NIK expression was investigated during the improvement of insulin resistance.</p><p><b>RESULTS</b>NIK was increased by about 1-fold in the renal tissues of Lep(ob) mice and C57BL/6J mice fed a HFD for 12 weeks. After 1 and 3 weeks of high-fat feeding, we observed an almost 50% decrease in NIK and IKKα expression in the liver and renal tissues of C57BL/6J mice. NIK expression was significantly lower in the liver and renal tissues of HFD-fed mice that were treated with insulin sensitizers, metformin and sulfasalazine. However, IKKα expression was increased after metformin treatment in both tissues.</p><p><b>CONCLUSION</b>These results suggest a possible role of NIK in the liver and renal tissues of insulin-resistant mice.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Sang
/
Poids
/
Insulinorésistance
/
Technique de Western
/
Jeûne
/
Protein-Serine-Threonine Kinases
/
I-kappa B Kinase
/
Hyperglycémie provoquée
/
Insuline
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Medical Journal
Année:
2011
Type:
Article
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