Sequence Analysis of A Novel HLA-B*13:68 Allele / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this research was to identify a novel HLA-B allele in Chinese population.</p><p><b>METHODS</b>The HLA typing of bone marrow donors was performed by PCR-SBT. The ambiguous novel HLA allele was confirmed with GSSP method.</p><p><b>RESULTS</b>The sequence of a sample was different from all alleles in the HLA-B databases. The sequence analysis showed that it differed from the closet matching allele HLA-B*130101 at one nucleotide substitution, 137 T > C in Exon2, which resulted in an amino acid change from Phenylalanine (Phe) to Serine (Ser) at codon 22.</p><p><b>CONCLUSION</b>A novel allele was identified and named as HLA-B*1368 by the WHO Nomenclature Committee.</p>